13. GLaDOS (Portal)
https://www.youtube.com/watch?v=078hxx-JMJY Let's just admit the truth here; we all played the final boss battle in Portal constantly just to hear GLaDOS insult us again and loved every second of it. There's a disgruntled charm to the performance from Ellen McClain as GLaDOS, which keeps you smiling as she takes jabs at everything from your weight to your lack of parents. It's an interesting contrast as you try to kill the very being that is taking every opportunity to insult you, the kind of subversive brilliance that Valve is known for. The one part about the GLaDOS battle that works more than anything else is the fun of it all, where dragging mechanical nodes across a room to incinerate them has never been more satisfying. It's a tremendously
hilarious sequence that is a perfect cap to the already stellar game that came before it, asking the player to laugh and be a one-woman robot killing machine all at the same time.