16. Queen Bee
A femme fatale alien with crazy bees as minions, a female villain would be a great change in pace from the standard male big bad. Of course, Queen Bee would try invading the earth, similar to Loki in The Avengers, so you can argue whether or not it would be the best idea to use a similar plot again. However, one interesting storyline involving Queen Bee has her take over the H.I.V.E, and international terrorist group more notorious for their conflicts with the Teen Titans. The H.I.V.E. would make fascinating villains for a Justice League film, sending human henchmen out to undermine the Justice League behind the scenes, while Queen Bee invades the earth in the foreground. It would be a multi-layered conflict, with numerous threats coming in from all directions. The Justice League would be divided, and forced to question what to do next. A really complicated and sticky situation.