43. The Governor
FIRST APPEARANCE: #27 (2006) Though lacking the supernatural abilities of many villains on this list, The Governor is a vicious and detestable as grounded, real-life villains come. When Rick Grimes and co. are exploring the town of Woodbury, they meet The Governor, who first appears to be amenable and welcoming, but soon enough reveals for darker intentions. The Governor wishes to take Rick's prison settlement for his own, and chops off his hand in an effort to find it out. In addition to this, he has Michonne raped, which results in her severely disfiguring him in revenge. Eventually, The Governor's despotic attitude leads to his own Woodbury Army turning on him, leaving him dead and ready to be feasted on by the Walkers. In the TV series - which departs somewhat from the comic book depiction - he is played with searing brilliance by David Morrissey.
42. Thunderbolt Ross
FIRST APPEARANCE: INCREDIBLE HULK #1 (1962) Thaddeus "Thunderbolt" Ross isn't just a U.S. Army General, but he's also the father of Bruce Banner's love interest, Betty Ross. He was the head of the very Gamma Bomb Project that eventually caused Banner to become the Hulk, a creature that Thaddeus became totally obsessed with capturing (and upon discovering the Hulk's human identity, his determination seemed to grow even more intensely). In 2008, a new iteration of the Hulk was debuted, a red version whom the identity of which remained secret for some time. It was eventually revealed that Thaddeus was himself the monster, a desperate move to give himself a better chance at bringing the Hulk down once and for all. Though the Red Hulk has been met with a mixed response from fans and critics, it's a bold direction in which to take the character, and further cements just how obsessed the man is with stopping the beast. Besides, his being distinguished from the hulk so obviously - with his enhanced tactical skill and abilities, such as heat vision - help to make him a palpable, authentic threat.
41. Kraven The Hunter
FIRST APPEARANCE: THE AMAZING SPIDER-MAN #15 (1964) Kraven the Hunter may not be one of Spider-Man's more iconic foes, but Kraven's alter-ego, Sergei Kravinoff, is the half-brother of Dmitri Smerdyakov aka the Chameleon. Kraven has many of the same physical advantages as Spidey, though they are not quite as developed; his main win is that he can incur absurd amounts of damage, and also ages extremely slowly. The potions he drinks for physical acuity extend to allowing him to track his "prey" like an animal would, and he has an extensive knowledge of human anatomy, allowing him to methodically break down a human body in the most efficient way possible. One of the most bizarre aspects of his character is that, due to the ceremony that brought him back to life from his suicide, he can apparently only be killed by Spider-Man. This adds a certain definitive appeal to his battles with Spider-Man, for his battles with anyone else are essentially rendered meaningless (given that he has no chance of dying).