46. Lizard
FIRST APPEARANCE: THE AMAZING SPIDER-MAN VOL. 1, #6 (1963) Before he came The Lizard, Curt Connors was a genetic biologist whose research primarily involved attempts to re-grow human limbs by studying lizard DNA. However, testing this on his own missing arm caused him to mutate into a gigantic, hulking lizard monster, with little of the man underneath remaining. In addition to super-strength and speed, Lizard's thick, scaly skin allows him to withstand conventional attack forms from Spider-Man, and much like a regular lizard, his limbs can regrow after being detached. In addition to this, his tail can act like a powerful whip, and his teeth are capable of easy killing any human they chomp into. One major weakness of Lizard is that his mental functions are severely impaired, driven primarily by murderous rage and lacking the sure genius of his human counterpart. Even when he attempts to comprehend Connors' research, he lacks the emotional intelligence needed to put it to proper use. Connors was played by Dylan Baker in Sam Raimi's Spider-Man movies, and then by Rhys Ifans in The Amazing Spider-Man, where we actually saw him become The Lizard.
45. Hobgoblin
FIRST APPEARANCE: THE AMAZING SPIDER-MAN #238 (1983) The Hobgoblin was created when comic book writers didn't want to have to bring the Green Goblin back from the dead or resort to tired plot strands, so created a new spin on the character by way of this dangerous new supervillain, whose identity was not revealed for a long time. Roderick Kingsley turned out to be the first Hobgoblin, and was smart enough to reconstitute the Green Goblin formula from Norman Osborne's incomplete notes and also improve it. Essentially an enhanced version of the GG in every way, he also had a vast network of criminals in his employ, while not being burdened with the same brand of insanity that held Osborne back. The one problem with Hobgoblin is his egomaniacal nature; he is extremely proud and arrogant, to the point that he has ended up inadvertently revealing his own identity as a result. Though perhaps not as iconic as Osborne's character, he is in most ways a more vicious and intelligent counterpart.
44. The Abomination
FIRST APPEARANCE: TALES TO ASTONISH #90 (1967) KBH Agent Emil Blonsky became The Abomination by subjecting himself to an even higher dose of gamma radiation than Bruce Banner had been exposed to, transforming him into essentially a stronger, more scaly and intimidating version of The Hulk. He is perhaps most famous for defeating The Hulk in their first battle due to his increased strength, though unlike the big green rage monster, his size does not adjust depending on his anger, nor can he return to his human form. However, unlike The Hulk, Abomination retains his human intelligence, can breathe underwater and even survive without oxygen, Furthermore, in later iterations, was even capable of breathing fire. He was portrayed by Tim Roth in the movie The Incredible Hulk.