1. The Joker (Heath Ledger)
He had to be in here, didn't he? The late Heath Ledger's performance as Batman's insane arch-enemy earned him a posthumous Academy Award for Best Performance by an Actor in a Supporting Role. Such was Ledger's commitment to this role, he apparently prepared himself for it by locking himself away for weeks on end, looking in to a mirror and perfecting the character traits that he wished to convey. The result was a much darker, entirely different looking Joker to anything we'd seen before, and it worked brilliantly. It's such an enormous shame that his tragic, early death means we'll never get to see it again.
Quote:"You wanna know how I got these scars? My father was a drinker and a fiend. And one night he goes off crazier than usual. Mommy gets the kitchen knife to defend herself. He doesn't like that. Not. One. Bit. So, me watching, he takes the knife to her, laughing while he does it. He turns to me and says, "Why so serious?" Comes at me with the knife. "WHY SO SERIOUS?" He sticks the blade in my mouth... "Let's put a smile on that face." And... Why so serious?" Honourable mentions for Doctor Octopus (Alfred Molina), Ra's Al Ghul (Liam Neeson), Red Skull (Hugo Weaving), Mystique (Rebecca Romijn), The Riddler (Jim Carrey), Ozymandias (Matthew Goode) and The Joker (Jack Nicholson). And now for the depictions that were pretty damn awful...